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(C) Spectrophotometric determination of                photometer.
          phenolic content in  tea extracts                      (D) Thin layer chromatography
          -  Prepare the standard curve using 10 µl of known     - A fixed amount of (5 µl) of each tea extract was taken
          amounts (0-50 µg) of Gallic acid.                      and  spotted  in  a  TLC  sheet    and  air  dried  in  room
          - Added 10 µl each of  unknown tea sample extract to   temperature
          an appropriately labelled test tube  (in triplicates).  - TLC plate was kept in a beaker containing ca. 100 ml
          - Added 1.25 ml of  Folin&Ciocalteu’s reagent and 1    solvent  [ petroleum ether: ethyl acetate (7:3)]
          ml sodium carbonate to each tube and mixed well.       - Setup kept aside for separation for 30 minutes
          -  Incubated at room temperature for at 1 hour and     - TLC sheet was taken out and the bands were
          absorbance measured at 765 nm in a UV-VIS Spectro-     visualized

          4. Observation & Results                                      (B) Spectrophotometer determination of gallic

                                                                        acid standard
          (A) Antimicrobial activity of methanol extract
          of different tea samples







                                                                        (C) Estimation of total phenolic content in tea extract








            CONCLUSION: Fresh tea leaves extract exhibited greater antimicrobial activity,   CONCLUSION: Fresh tea leaves have the highest accumulation of
            as evident by larger zones of inhibition against the tested bacteria followed by green tea.  total phenolic  content  followed by Green tea.

           (D) Thin Layer Chromatography of tea extract           phase.
                                                                  - According to the number of components (polyphenols)

                                           -  The  presence  of   in tea extract, the bands were clearly visible
                                           phytochemicals    in   1-Processed Tea
                                           tea  extracts  were    2-Green Tea
                                           analyzed  by  thin     3- Seabuckthorn Tea
                                           layer     chromato-    4- Fresh tea leaves
                                           graphic method. The
                                           solvents  used  were   CONCLUSION: Fresh tea leaves (4) and Green Tea (2)
                                           petroleum ether and    show two prominent bands, and correlates well with the highest
             � 1     � 2     3          4  ethyl  acetate  (7:3   phenolic  content  .  Hence  these  tea  are  more  beneficial  and
                                           ratio)   as   mobile   healthy to consume.





            References- https://www.ncbi.nlm.nih.gov/pmc/articles/P-                Yamini Bisht
            MC3845987/,https://chembioagro.springeropen.com/articles/10.1186/s40538-014-0028-7, https://www.re-  PGT , Biology,
            searchgate.net/publication/290797389_Phytochemi-                         Sadhu Vaswani
            cal_analysis_and_antimicrobial_activity_of_some_medicinal_plants_against_selected_common_human_pathog  International School
            enic_microorganisms
                     �
       30  xÉ<Ç =cÉxÉ fnlacj  -2023











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